Hotspot

accD-psaI

LSC · 60865-61305 · 441 bp

Back to Conioselinum

Pi 0.0114

MarkerSeek score 46.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 15
pi: 0.0114
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	AAAGAAGCCATTGCCATTGC	50.0	45.0	58.8	57.9	556-567 bp	1.0	76.5
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCATTGCCGGAAATACT	50.0	45.0	58.8	58.2	546-557 bp	1.0	76.2
accD-psaI_p3	GCTCCACGCTTTCTTTCCT	AAAGAAGCCATTGCCATTGC	52.6	45.0	58.1	57.9	556-567 bp	1.0	74.1
accD-psaI_p4	TCCACGCTTTCTTTCCTTTGA	AAAGAAGCCATTGCCATTGC	42.9	45.0	58.1	57.9	554-565 bp	1.0	74.0
accD-psaI_p5	GAGTTATTTCAGCTCCACGC	AAAGAAGCCATTGCCATTGC	50.0	45.0	56.9	57.9	567-578 bp	1.0	73.8