Hotspot

atpF-atpH

LSC · 13807-14104 · 298 bp

Back to Conioselinum

Pi 0.0155

MarkerSeek score 54.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 298
variable_sites: 14
pi: 0.0155
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	413-479 bp	1.0	80.1
atpF-atpH_p2	CCAGTGGCCTAAAGAAACGA	GCCTGGTTGTAGCATTAGCA	50.0	50.0	57.8	58.2	386-452 bp	1.0	79.8
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	415-481 bp	1.0	78.7
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.9	57.6	484-550 bp	1.0	78.3
atpF-atpH_p5	CCAGTGGCCTAAAGAAACGA	TGAGGCGGAGGGAAAAATAC	50.0	50.0	57.8	57.6	457-523 bp	1.0	78.0