Hotspot

trnP-GGG-trnL-UAG

Genome · 111359-111627 · 269 bp

Back to Coniogramme

Pi 0.123

MarkerSeek score 78.80

Primer available yes

Label trnP-GGG-trnL-UAG

Feature Metadata

label_name: trnP-GGG-trnL-UAG
feature_type: igs
region: Genome
length_bp: 269
variable_sites: 33
pi: 0.123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-trnL-UAG_p1	GCTACCAAACTGCGCCATAT	CTCTGGCACTGCTTCCTAAG	50.0	55.0	58.7	58.0	345 bp	1.0	82.2
trnP-GGG-trnL-UAG_p2	GCTACCAAACTGCGCCATAT	CTGGCACTGCTTCCTAAGAG	50.0	55.0	58.7	58.0	343 bp	1.0	82.2
trnP-GGG-trnL-UAG_p3	GCTACCAAACTGCGCCATAT	CAGCGTGTCTACCCATTTCA	50.0	50.0	58.7	57.9	323 bp	1.0	82.0
trnP-GGG-trnL-UAG_p4	GCTACCAAACTGCGCCATAT	AGCGTGTCTACCCATTTCAC	50.0	50.0	58.7	57.9	322 bp	1.0	82.0
trnP-GGG-trnL-UAG_p5	GACCTCCATCACCCCAAGAT	CTGGCACTGCTTCCTAAGAG	55.0	55.0	58.8	58.0	368 bp	1.0	81.0