Hotspot

psaJ-rpl33

Genome · 63485-63717 · 233 bp

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Pi 0.0644

MarkerSeek score 82.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 233
variable_sites: 15
pi: 0.0644
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ATATCTCTCTACGGCCCCTG	ACCATCAGTCTCTCTCCGAG	55.0	55.0	57.8	57.9	420-424 bp	1.0	80.6
psaJ-rpl33_p2	ATATCTCTCTACGGCCCCTG	CCATCAGTCTCTCTCCGAGT	55.0	55.0	57.8	57.9	419-423 bp	1.0	80.6
psaJ-rpl33_p3	ATATGGTTCGGCTTACTGGC	ACCATCAGTCTCTCTCCGAG	50.0	55.0	57.7	57.9	389-393 bp	1.0	80.6
psaJ-rpl33_p4	ATATGGTTCGGCTTACTGGC	CCATCAGTCTCTCTCCGAGT	50.0	55.0	57.7	57.9	388-392 bp	1.0	80.6
psaJ-rpl33_p5	GCTTACTGGCTGGTTCACTC	CCATCAGTCTCTCTCCGAGT	55.0	55.0	58.6	57.9	378-382 bp	1.0	78.3