Hotspot

trnP-UGG-psaJ

Genome · 63176-63304 · 129 bp

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Pi 0.0488

MarkerSeek score 77.20

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: Genome
length_bp: 129
variable_sites: 6
pi: 0.0488
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	CCCAAAACAAACACGCTACC	ATGTTGCATCGGAATCCTCC	50.0	50.0	57.9	58.0	220-228 bp	1.0	83.8
trnP-UGG-psaJ_p2	CCCAAAACAAACACGCTACC	GTTGCATCGGAATCCTCCTT	50.0	50.0	57.9	58.0	218-226 bp	1.0	83.7
trnP-UGG-psaJ_p3	CCAAAACAAACACGCTACCG	ATGTTGCATCGGAATCCTCC	50.0	50.0	58.3	58.0	219-227 bp	1.0	82.8
trnP-UGG-psaJ_p4	CCCAAAACAAACACGCTACC	GCATCGGAATCCTCCTTCTC	50.0	55.0	57.9	57.8	215-223 bp	1.0	82.8
trnP-UGG-psaJ_p5	CCAAAACAAACACGCTACCG	GTTGCATCGGAATCCTCCTT	50.0	50.0	58.3	58.0	217-225 bp	1.0	82.7