Hotspot

rps4-trnL-UAA

Genome · 44661-45396 · 736 bp

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Pi 0.053

MarkerSeek score 78.40

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 736
variable_sites: 38
pi: 0.053
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	GCAAGGCCTGGCAAATTTTT	ACGTCTCCATACCTAGGCTC	45.0	55.0	58.4	58.0	812-831 bp	1.0	82.3
rps4-trnL-UAA_p2	GCAAGGCCTGGCAAATTTTT	CGTCTCCATACCTAGGCTCA	45.0	55.0	58.4	58.0	811-830 bp	1.0	82.3
rps4-trnL-UAA_p3	GCAAGGCCTGGCAAATTTTT	TCTGCCATTTCGCCATATCC	45.0	50.0	58.4	58.1	771-790 bp	1.0	81.9
rps4-trnL-UAA_p4	GGCCTGGCAAATTTTTCAGA	ACGTCTCCATACCTAGGCTC	45.0	55.0	57.2	58.0	808-827 bp	1.0	78.8
rps4-trnL-UAA_p5	GGCCTGGCAAATTTTTCAGA	CGTCTCCATACCTAGGCTCA	45.0	55.0	57.2	58.0	807-826 bp	1.0	78.7