Hotspot

psbD-trnT-GGU

Genome · 33788-35593 · 1806 bp

Back to Coniogramme

Pi 0.0967

MarkerSeek score 84.90

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1806
variable_sites: 173
pi: 0.0967
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	TCTTCTAAGCCAGTCGTCCA	GCCCTTTTAACTCAGCGGTA	50.0	50.0	58.1	57.9	1953-1979 bp	1.0	86.3
psbD-trnT-GGU_p2	TCTTCTAAGCCAGTCGTCCA	AAGCCCTTTTAACTCAGCGG	50.0	50.0	58.1	58.5	1955-1981 bp	1.0	83.4
psbD-trnT-GGU_p3	CTCTTCTAAGCCAGTCGTCC	GCCCTTTTAACTCAGCGGTA	55.0	50.0	57.4	57.9	1954-1980 bp	1.0	82.3
psbD-trnT-GGU_p4	TCTTCTAAGCCAGTCGTCCA	CGGTGATAGCTAAGCCCTTT	50.0	50.0	58.1	57.4	1966-1992 bp	1.0	82.1
psbD-trnT-GGU_p5	TCTTCTAAGCCAGTCGTCCA	GCCATGGTAAGGCGTAAGTC	50.0	55.0	58.1	58.7	1926-1952 bp	1.0	81.6