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petN-trnC-GCA

Genome · 29219-29685 · 467 bp

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Pi 0.0568

MarkerSeek score 79.40

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: Genome
length_bp: 467
variable_sites: 26
pi: 0.0568
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	ACGTCACAACCCACTTCTTC	CCTCAATTCCCCAGTTCGAG	50.0	55.0	58.1	58.0	574-575 bp	1.0	84.2
petN-trnC-GCA_p2	ACGTCACAACCCACTTCTTC	TTGGGACGAGGCGATATAGT	50.0	50.0	58.1	58.0	621-622 bp	1.0	84.0
petN-trnC-GCA_p3	CAACCCACTTCTTCCCCAAA	CCTCAATTCCCCAGTTCGAG	50.0	55.0	57.9	58.0	568-569 bp	1.0	84.0
petN-trnC-GCA_p4	CAACCCACTTCTTCCCCAAA	TTGGGACGAGGCGATATAGT	50.0	50.0	57.9	58.0	615-616 bp	1.0	83.8
petN-trnC-GCA_p5	AACCCACTTCTTCCCCAAAC	TTGGGACGAGGCGATATAGT	50.0	50.0	57.9	58.0	614-615 bp	1.0	83.8