Hotspot

trnG-UCC-trnR-UCU

Genome · 9708-9898 · 191 bp

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Pi 0.126

MarkerSeek score 77.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: Genome
length_bp: 191
variable_sites: 24
pi: 0.126
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	TACTGATGCGGGTTCGATTC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.8	56.9	258-259 bp	1.0	69.6
trnG-UCC-trnR-UCU_p2	CTACTGATGCGGGTTCGATT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.8	56.9	259-260 bp	1.0	69.5
trnG-UCC-trnR-UCU_p3	TACTGATGCGGGTTCGATTC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	57.8	57.9	264-265 bp	1.0	68.7
trnG-UCC-trnR-UCU_p4	CTACTGATGCGGGTTCGATT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	57.8	57.9	265-266 bp	1.0	68.7
trnG-UCC-trnR-UCU_p5	CTTCCAAGCTACTGATGCGG	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.7	56.9	267-268 bp	1.0	65.4