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psbI-trnS-GCU

Genome · 7712-7811 · 100 bp

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Pi 0.153

MarkerSeek score 77.40

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 100
variable_sites: 15
pi: 0.153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATGATCCCGGACGAAATCC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.7	58.2	157-159 bp	1.0	84.8
psbI-trnS-GCU_p2	AATGATCCCGGACGAAATCC	AGAGGTCTAAAGCGTCGGAT	50.0	50.0	57.7	58.2	209-211 bp	1.0	82.5
psbI-trnS-GCU_p3	AATGATCCCGGACGAAATCC	AGGTCTAAAGCGTCGGATTG	50.0	50.0	57.7	57.7	207-209 bp	1.0	82.1
psbI-trnS-GCU_p4	AATGATCCCGGACGAAATCC	GTCTAAAGCGTCGGATTGCT	50.0	50.0	57.7	58.4	205-207 bp	1.0	81.7
psbI-trnS-GCU_p5	AATGATCCCGGACGAAATCC	GAGGTCTAAAGCGTCGGATT	50.0	50.0	57.7	57.4	208-210 bp	1.0	79.8