Hotspot

rrn16S

IRa · 144528-146027 · 1500 bp

Back to Commiphora

Pi 0.0048

MarkerSeek score 62.60

Primer available yes

Label rrn16S

Feature Metadata

label_name: rrn16S
feature_type: rRNA
region: IRa
length_bp: 1500
variable_sites: 18
pi: 0.0048
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S_p1	AGTTTCGTAAGGGGACTGGA	TGTACTCCACGGATAGAGGA	50.0	50.0	58.0	56.6	1667-1672 bp	1.0	69.5
rrn16S_p2	ATGATGCCATGTGAATCGCT	TGTACTCCACGGATAGAGGA	45.0	50.0	58.0	56.6	1724-1729 bp	1.0	69.5
rrn16S_p3	GCAGGCTACCATGAGACAAA	TGTACTCCACGGATAGAGGA	50.0	50.0	57.9	56.6	1647-1652 bp	1.0	68.8
rrn16S_p4	AGTTTCGTAAGGGGACTGGA	TGTACTCCACGGATAGAGGAA	50.0	47.6	58.0	57.3	1667-1672 bp	1.0	67.5
rrn16S_p5	ATGATGCCATGTGAATCGCT	TGTACTCCACGGATAGAGGAA	45.0	47.6	58.0	57.3	1724-1729 bp	1.0	67.5