Hotspot

rpoB

LSC · 25000-28212 · 3213 bp

Back to Commiphora

Pi 0.0105

MarkerSeek score 50.20

Primer available yes

Label rpoB

Feature Metadata

label_name: rpoB
feature_type: gene
region: LSC
length_bp: 3213
variable_sites: 75
pi: 0.0105
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB_p1	GATACCGGGGCTTTGCAATA	TCACTCACGTATTCTTCCGA	50.0	45.0	58.0	56.0	180 bp	1.0	69.0
rpoB_p2	CGAAAGCCCATTATGGTCCA	TCACTCACGTATTCTTCCGA	50.0	45.0	57.9	56.0	216 bp	1.0	67.9
rpoB_p3	ACCGAAAGCCCATTATGGTC	TCACTCACGTATTCTTCCGA	50.0	45.0	57.9	56.0	218 bp	1.0	67.9
rpoB_p4	GATACCGGGGCTTTGCAATA	TCACTCACGTATTCTTCCGAA	50.0	42.9	58.0	56.8	180 bp	1.0	67.1
rpoB_p5	AATAGATACCGGGGCTTTGC	TCACTCACGTATTCTTCCGA	50.0	45.0	57.7	56.0	184 bp	1.0	66.9