Hotspot

ndhH

SSC · 126178-127359 · 1182 bp

Back to Commiphora

Pi 0.0188

MarkerSeek score 64.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 53
pi: 0.0188
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	GACAAGCATCCATATGATCCCA	TGAACTTCTTTTCGTTTTCAACA	45.5	30.4	58.0	55.8	1330-1331 bp	1.0	43.2
ndhH_p2	AGGGACAAGCATCCATATGA	TGAACTTCTTTTCGTTTTCAACA	45.0	30.4	56.0	55.8	1333-1334 bp	1.0	43.2
ndhH_p3	GACAAGCATCCATATGATCCCA	TCATGTAAGAATGAATCGAGGA	45.5	36.4	58.0	54.7	1305-1306 bp	1.0	43.2
ndhH_p4	AGGGACAAGCATCCATATGA	TCATGTAAGAATGAATCGAGGA	45.0	36.4	56.0	54.7	1308-1309 bp	1.0	43.2
ndhH_p5	ACAAGCATCCATATGATCCCA	TGAACTTCTTTTCGTTTTCAACA	42.9	30.4	56.8	55.8	1329-1330 bp	1.0	43.2