Hotspot

ccsA

SSC · 118480-119448 · 969 bp

Back to Commiphora

Pi 0.00826

MarkerSeek score 53.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 18
pi: 0.00826
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TCCATTCCGTATACTTGAGGAAC	CAGGCCCTTTCCTTTCTTCA	43.5	50.0	57.7	57.7	1045-1046 bp	1.0	52.9
ccsA_p2	CCATTCCGTATACTTGAGGAACT	CAGGCCCTTTCCTTTCTTCA	43.5	50.0	57.7	57.7	1044-1045 bp	1.0	52.8
ccsA_p3	TCCATTCCGTATACTTGAGGA	CAGGCCCTTTCCTTTCTTCA	42.9	50.0	55.6	57.7	1045-1046 bp	1.0	51.8
ccsA_p4	TCCATTCCGTATACTTGAGGAAC	TCATCAGGCCCTTTCCTTTC	43.5	50.0	57.7	57.5	1049-1050 bp	1.0	51.1
ccsA_p5	CCATTCCGTATACTTGAGGAACT	TCATCAGGCCCTTTCCTTTC	43.5	50.0	57.7	57.5	1048-1049 bp	1.0	51.0