Hotspot

ndhD-psaC

SSC · 121286-121434 · 149 bp

Back to Commiphora

Pi 0.0517

MarkerSeek score 72.80

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 149
variable_sites: 19
pi: 0.0517
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	TTAAAGAACCCGCGGCTATC	AAACAACTCGAAGCATGGGT	50.0	45.0	58.1	58.0	259-261 bp	1.0	85.9
ndhD-psaC_p2	TTAAAGAACCCGCGGCTATC	GGCATGAAACAACTCGAAGC	50.0	50.0	58.1	58.0	265-267 bp	1.0	85.6
ndhD-psaC_p3	AAAATTAAAGAACCCGCGGC	AAACAACTCGAAGCATGGGT	45.0	45.0	57.6	58.0	263-265 bp	1.0	83.4
ndhD-psaC_p4	AAAATTAAAGAACCCGCGGC	GGCATGAAACAACTCGAAGC	45.0	50.0	57.6	58.0	269-271 bp	1.0	83.1
ndhD-psaC_p5	TTAAAGAACCCGCGGCTATC	AAGAGATGTGAATCCGCCTG	50.0	50.0	58.1	57.7	320-322 bp	1.0	82.2