Hotspot

ccsA-ndhD

SSC · 119449-119764 · 316 bp

Back to Commiphora

Pi 0.0234

MarkerSeek score 61.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 316
variable_sites: 17
pi: 0.0234
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGATGGTAGTAGGCTACGCA	TTATCGATGGCTTCCCTTGC	50.0	50.0	57.9	58.0	1214-1227 bp	1.0	82.3
ccsA-ndhD_p2	TGATGGTAGTAGGCTACGCA	GCTGGCTATCCCAATTCCAA	50.0	50.0	57.9	57.9	1257-1270 bp	1.0	81.9
ccsA-ndhD_p3	TGATGGTAGTAGGCTACGCA	GCATATGGGTTGGTTCGGAT	50.0	50.0	57.9	58.0	1584-1597 bp	1.0	81.8
ccsA-ndhD_p4	TGATGGTAGTAGGCTACGCA	TACAGGACTTAACGGAGCCA	50.0	50.0	57.9	58.1	1381-1394 bp	1.0	81.7
ccsA-ndhD_p5	TGTGGCTTCTATGGGCTTTC	GCATATGGGTTGGTTCGGAT	50.0	50.0	57.9	58.0	1133-1146 bp	1.0	81.3