Hotspot

trnN-GUU-ndhF

IRb · 112878-114333 · 1456 bp

Back to Commiphora

Pi 0.0193

MarkerSeek score 62.00

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1456
variable_sites: 68
pi: 0.0193
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GAACTTGGGTGTGGGTCTTT	ATATGTAGGGGGAAGTCGCA	50.0	50.0	57.9	57.9	2120-2159 bp	1.0	83.3
trnN-GUU-ndhF_p2	GAACTTGGGTGTGGGTCTTT	TATGTAGGGGGAAGTCGCAT	50.0	50.0	57.9	57.9	2119-2158 bp	1.0	83.3
trnN-GUU-ndhF_p3	GAACTTGGGTGTGGGTCTTT	GGGGAAGTCGCATCTCTTTT	50.0	50.0	57.9	57.9	2112-2151 bp	1.0	83.2
trnN-GUU-ndhF_p4	CTATGGAACTTGGGTGTGGG	TATGTAGGGGGAAGTCGCAT	55.0	50.0	57.9	57.9	2124-2163 bp	1.0	82.8
trnN-GUU-ndhF_p5	GAACTTGGGTGTGGGTCTTT	TCCTTTGTTTGTTGGAGCCA	50.0	45.0	57.9	57.8	2589-2628 bp	1.0	82.7