Hotspot

petA-psbJ

LSC · 65846-66965 · 1120 bp

Back to Commiphora

Pi 0.00708

MarkerSeek score 66.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1120
variable_sites: 16
pi: 0.00708
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.0	57.4	957-1269 bp	1.0	80.0
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	TATTCCGGATTGGGTTCGTC	50.0	50.0	58.2	57.4	954-1266 bp	1.0	78.8
petA-psbJ_p3	ATCCATTACGTGTCCAAGGC	GGATTGGGTTCGTCCTTGTA	50.0	50.0	58.0	57.2	951-1263 bp	1.0	78.6
petA-psbJ_p4	CATTACGTGTCCAAGGCCTT	GGATTGGGTTCGTCCTTGTA	50.0	50.0	58.2	57.2	948-1260 bp	1.0	77.5
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	TATTCCGGATTGGGTTCGTC	45.0	50.0	57.6	57.4	952-1264 bp	1.0	77.5