Hotspot

ndhK-ndhC

LSC · 52835-52895 · 61 bp

Back to Commiphora

Pi 0.0377

MarkerSeek score 71.10

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 61
variable_sites: 4
pi: 0.0377
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	CTGGAGAGCCTTGACCAATT	AAAGGAGCATTGGAGTGGTC	50.0	50.0	57.5	57.8	180-184 bp	1.0	77.4
ndhK-ndhC_p2	ACTGGAGAGCCTTGACCAAT	AAAGGAGCATTGGAGTGGTC	50.0	50.0	58.6	57.8	181-185 bp	1.0	76.3
ndhK-ndhC_p3	CTGGAGAGCCTTGACCAATT	CAGTTTATGCGTGGCGAAAA	50.0	45.0	57.5	57.7	197-201 bp	1.0	76.2
ndhK-ndhC_p4	CTGGAGAGCCTTGACCAATT	GTTTATGCGTGGCGAAAAGG	50.0	50.0	57.5	58.4	195-199 bp	1.0	75.7
ndhK-ndhC_p5	ACTGGAGAGCCTTGACCAAT	CAGTTTATGCGTGGCGAAAA	50.0	45.0	58.6	57.7	198-202 bp	1.0	75.1