Hotspot

trnD-GUC-trnY-GUA

LSC · 31633-32126 · 494 bp

Back to Commiphora

Pi 0.00923

MarkerSeek score 68.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 494
variable_sites: 9
pi: 0.00923
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	CGGTGCTCTGACCAATTGAA	TTTTCTTCCTGGGTCGATGC	50.0	50.0	58.5	58.2	574-618 bp	1.0	79.9
trnD-GUC-trnY-GUA_p2	CGGTGCTCTGACCAATTGAA	TGGCAATATGTCTACGCTGG	50.0	50.0	58.5	57.8	520-564 bp	1.0	79.6
trnD-GUC-trnY-GUA_p3	CGGTGCTCTGACCAATTGAA	GGCAATATGTCTACGCTGGT	50.0	50.0	58.5	57.8	519-563 bp	1.0	79.6
trnD-GUC-trnY-GUA_p4	CGGTGCTCTGACCAATTGAA	GGGGACGGACTGTAAATTCG	50.0	55.0	58.5	58.4	541-585 bp	1.0	78.6
trnD-GUC-trnY-GUA_p5	CGGTGCTCTGACCAATTGAA	GGGACGGACTGTAAATTCGT	50.0	50.0	58.5	57.6	540-584 bp	1.0	78.4