Hotspot

atpF-atpH

LSC · 14523-15058 · 536 bp

Back to Commiphora

Pi 0.00871

MarkerSeek score 69.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 536
variable_sites: 10
pi: 0.00871
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CCAGTGAACCAAGGAAACGA	CCGAGGCAGAGGGAAAAATA	50.0	50.0	58.0	57.3	677-704 bp	1.0	78.3
atpF-atpH_p2	CCAGTGAACCAAGGAAACGA	TGGACTGGTTGTAGCATTAGC	50.0	47.6	58.0	58.0	607-634 bp	1.0	76.3
atpF-atpH_p3	CCAGTGAACCAAGGAAACGA	GCAGAGGGAAAAATACGAGGT	50.0	47.6	58.0	58.0	672-699 bp	1.0	75.9
atpF-atpH_p4	CAAATGGCCAGTGAACCAAG	CCGAGGCAGAGGGAAAAATA	50.0	50.0	57.6	57.3	684-711 bp	1.0	75.0
atpF-atpH_p5	CCAGTGAACCAAGGAAACGA	GAGGCAGAGGGAAAAATACGA	50.0	47.6	58.0	57.7	675-702 bp	1.0	73.9