Hotspot

trnH-GUG-psbA

LSC · 130-548 · 419 bp

Back to Commiphora

Pi 0.0311

MarkerSeek score 70.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 23
pi: 0.0311
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TTCCCTCTAGACCTAGCTGC	55.0	55.0	58.1	57.9	479-487 bp	1.0	83.5
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	493-501 bp	1.0	83.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	492-500 bp	1.0	83.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	494-502 bp	1.0	83.3
trnH-GUG-psbA_p5	ATGGTAGTTATGGGCGAACG	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.8	57.9	544-552 bp	1.0	82.1