Hotspot

psbD

LSC · 32549-33610 · 1062 bp

Back to Commidendrum

Pi 0

MarkerSeek score 25.80

Primer available yes

Label psbD

Feature Metadata

label_name: psbD
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD_p1	CAGGTCTGAAGATCAACCGG	CTAACAATCCAGCCTTCCCC	55.0	55.0	58.0	57.9	2023 bp	1.0	79.4
psbD_p2	ATGATCGACTCTTTGGACGC	CTAACAATCCAGCCTTCCCC	50.0	55.0	58.1	57.9	1764 bp	1.0	79.0
psbD_p3	CAGGTCTGAAGATCAACCGG	GCCCAATACTGCAGAGGAAA	55.0	50.0	58.0	57.9	1724 bp	1.0	78.9
psbD_p4	CAGGTCTGAAGATCAACCGG	AAAGCCCAATACTGCAGAGG	55.0	50.0	58.0	57.9	1727 bp	1.0	78.8
psbD_p5	GGGGTAGTGCACGAGAAAAA	CTAACAATCCAGCCTTCCCC	50.0	55.0	58.1	57.9	1797 bp	1.0	78.7