Hotspot

ycf4-cemA

LSC · 60358-61263 · 906 bp

Back to Commidendrum

Pi 0.000552

MarkerSeek score 45.10

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 906
variable_sites: 1
pi: 0.000552
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1086 bp	1.0	79.2
ycf4-cemA_p2	CTCCACGGGAAATGGAACAA	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.1	58.1	1050 bp	1.0	78.7
ycf4-cemA_p3	GAGCCATTCCATTGACTCGT	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.0	57.6	1085 bp	1.0	76.7
ycf4-cemA_p4	CTCCACGGGAAATGGAACAA	AGAAATCCACCAGGGCAAAA	50.0	45.0	58.1	57.6	1049 bp	1.0	76.1
ycf4-cemA_p5	GGAGCCATTCCATTGACTCG	GAGAAATCCACCAGGGCAAA	55.0	50.0	58.7	58.1	1087 bp	1.0	73.9