Hotspot

ndhC-trnV-UAC

LSC · 50495-51266 · 772 bp

Back to Commidendrum

Pi 0.000648
MarkerSeek score 45.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
772
variable_sites
1
pi
0.000648
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GTCCTTTGCTAATCGGAGCT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 917-918 bp 1.0 76.0
ndhC-trnV-UAC_p2 CGGTCCTTTGCTAATCGGAG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 919-920 bp 1.0 75.1
ndhC-trnV-UAC_p3 CTCCGGTCCTTTGCTAATCG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.4 57.8 922-923 bp 1.0 75.1
ndhC-trnV-UAC_p4 GTCCTTTGCTAATCGGAGCT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 916-917 bp 1.0 73.2
ndhC-trnV-UAC_p5 GGTCCTTTGCTAATCGGAGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.7 57.8 918-919 bp 1.0 72.9