Hotspot

rpl16

LSC · 85410-86932 · 1523 bp

Back to Commelina

Pi 0.0151

MarkerSeek score 56.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1523
variable_sites: 33
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGGCGCCTATGATTCGAATA	TTGAAGGAATCGCACGCATA	50.0	45.0	58.0	58.0	2360-2397 bp	1.0	82.7
rpl16_p2	CGGCGCCTATGATTCGAATA	GGTTTTGAAGGAATCGCACG	50.0	50.0	58.0	58.0	2364-2401 bp	1.0	82.5
rpl16_p3	ATCAATTTTCGAGCTCCGCT	TTGAAGGAATCGCACGCATA	45.0	45.0	58.0	58.0	2337-2374 bp	1.0	82.5
rpl16_p4	CGAGCAATCGGACCAAAAAC	TTGAAGGAATCGCACGCATA	50.0	45.0	58.0	58.0	2589-2626 bp	1.0	82.3
rpl16_p5	GCAATCGGACCAAAAACTCG	TTGAAGGAATCGCACGCATA	50.0	45.0	58.0	58.0	2586-2623 bp	1.0	82.3