Hotspot

ndhK

LSC · 52392-53120 · 729 bp

Back to Commelina

Pi 0.00457

MarkerSeek score 61.70

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 729
variable_sites: 5
pi: 0.00457
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	CGATCCTGCTGCATCTTCTT	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.0	58.1	855 bp	1.0	79.4
ndhK_p2	AAACGATCCTGCTGCATCTT	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.9	58.1	858 bp	1.0	78.8
ndhK_p3	CGATCCTGCTGCATCTTCTT	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	943 bp	1.0	78.4
ndhK_p4	AAACGATCCTGCTGCATCTT	TGGGCAATGAGTTTCGATGT	45.0	45.0	57.9	57.8	946 bp	1.0	77.9
ndhK_p5	AACGATCCTGCTGCATCTTC	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.3	58.1	857 bp	1.0	77.1