Hotspot

clpP1

LSC · 73326-76142 · 2817 bp

Back to Commelina

Pi 0.0151

MarkerSeek score 55.20

Primer available yes

Label clpP1

Feature Metadata

label_name: clpP1
feature_type: gene
region: LSC
length_bp: 2817
variable_sites: 63
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP1_p1	TCGAATAGGTTGGTTTAGATCGA	AAATCATTGCCGCGTTTACG	39.1	45.0	57.2	58.1	2994 bp	0.333333	21.7
clpP1_p2	TTCGAATAGGTTGGTTTAGATCGA	AAATCATTGCCGCGTTTACG	37.5	45.0	57.8	58.1	2995 bp	0.333333	18.8
clpP1_p3	CGAATAGGTTGGTTTAGATCGA	AAATCATTGCCGCGTTTACG	40.9	45.0	55.7	58.1	2993 bp	0.333333	18.4
clpP1_p4	TCGAATAGGTTGGTTTAGATCG	AAATCATTGCCGCGTTTACG	40.9	45.0	55.7	58.1	2994 bp	0.333333	18.4
clpP1_p5	TCGAATAGGTTGGTTTAGATCGAT	AAATCATTGCCGCGTTTACG	37.5	45.0	57.6	58.1	2994 bp	0.333333	17.2