Hotspot

psaC-ndhE

SSC · 122147-122701 · 555 bp

Back to Commelina

Pi 0.0337

MarkerSeek score 68.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 555
variable_sites: 28
pi: 0.0337
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	GTCCGTCAATTCCCAGTCTC	GCAGCCATTGGGTTAGCTAT	55.0	50.0	58.0	58.0	1264-1283 bp	1.0	83.3
psaC-ndhE_p2	CGTCAATTCCCAGTCTCCAG	GCAGCCATTGGGTTAGCTAT	55.0	50.0	58.0	58.0	1261-1280 bp	1.0	83.3
psaC-ndhE_p3	TGGCCAAGCTGCTAAAGTAG	GCAGCCATTGGGTTAGCTAT	50.0	50.0	57.9	58.0	1329-1348 bp	1.0	82.4
psaC-ndhE_p4	GTCCGTCAATTCCCAGTCTC	AGACACATCCTGGTACGGAA	55.0	50.0	58.0	58.1	1916-1935 bp	1.0	81.9
psaC-ndhE_p5	CGTCAATTCCCAGTCTCCAG	AGACACATCCTGGTACGGAA	55.0	50.0	58.0	58.1	1913-1932 bp	1.0	81.9