Hotspot

rpl32-trnL-UAG

SSC · 118081-118835 · 755 bp

Back to Commelina

Pi 0.0339
MarkerSeek score 71.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
755
variable_sites
37
pi
0.0339
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGTGCGACAAACAAACACAA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.3 57.9 832-839 bp 1.0 78.6
rpl32-trnL-UAG_p2 TTGTGCGACAAACAAACACA TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.3 57.9 833-840 bp 1.0 78.6
rpl32-trnL-UAG_p3 TGTGCGACAAACAAACACAA AGCAGCGTGTCTACCAATTT 40.0 45.0 57.3 57.8 825-832 bp 1.0 78.2
rpl32-trnL-UAG_p4 TTGTGCGACAAACAAACACA AGCAGCGTGTCTACCAATTT 40.0 45.0 57.3 57.8 826-833 bp 1.0 78.2
rpl32-trnL-UAG_p5 TGTGCGACAAACAAACACAA CTAAGAGCAGCGTGTCTACC 40.0 55.0 57.3 57.8 830-837 bp 1.0 78.1