Hotspot

rpl14-rpl16

LSC · 85288-85409 · 122 bp

Back to Commelina

Pi 0.041

MarkerSeek score 68.40

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 7
pi: 0.041
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	ATCAATTTTCGAGCTCCGCT	AATAGCTGCGTCCAAAATGC	45.0	45.0	58.0	57.4	233-239 bp	1.0	77.6
rpl14-rpl16_p2	CGGCGCCTATGATTCGAATA	AATAGCTGCGTCCAAAATGC	50.0	45.0	58.0	57.4	256-262 bp	1.0	77.5
rpl14-rpl16_p3	TTTCGAGCTCCGCTGTTATC	AATAGCTGCGTCCAAAATGC	50.0	45.0	58.1	57.4	227-233 bp	1.0	77.3
rpl14-rpl16_p4	ATTACCGGCGCCTATGATTC	AATAGCTGCGTCCAAAATGC	50.0	45.0	57.9	57.4	261-267 bp	1.0	76.8
rpl14-rpl16_p5	GATTACCGGCGCCTATGATT	AATAGCTGCGTCCAAAATGC	50.0	45.0	57.9	57.4	262-268 bp	1.0	76.8