Hotspot

rpl33-rps18

LSC · 71255-71407 · 153 bp

Back to Commelina

Pi 0.0218

MarkerSeek score 65.40

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 5
pi: 0.0218
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	GACACAATACGTCTGGTCGA	GTGCAAACGCCTACGAAAAG	50.0	50.0	57.4	58.3	299-332 bp	1.0	73.7
rpl33-rps18_p2	TCGACACAATACGTCTGGTC	GTGCAAACGCCTACGAAAAG	50.0	50.0	57.4	58.3	301-334 bp	1.0	73.7
rpl33-rps18_p3	GACACAATACGTCTGGTCGA	TGCAAACGCCTACGAAAAGA	50.0	45.0	57.4	58.4	298-331 bp	1.0	73.0
rpl33-rps18_p4	TCGACACAATACGTCTGGTC	TGCAAACGCCTACGAAAAGA	50.0	45.0	57.4	58.4	300-333 bp	1.0	73.0
rpl33-rps18_p5	CGACACAATACGTCTGGTCG	GTGCAAACGCCTACGAAAAG	55.0	50.0	58.7	58.3	300-333 bp	1.0	72.7