Hotspot

trnC-GCA-petN

LSC · 29738-30730 · 993 bp

Back to Commelina

Pi 0.0108

MarkerSeek score 64.00

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 993
variable_sites: 16
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GACATAGCCAAGCGGTAAGG	TACCCCTAGAGTCCACTCCT	55.0	55.0	58.4	58.0	1153-1156 bp	1.0	78.7
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	TACCCCTAGAGTCCACTCCT	50.0	55.0	58.5	58.0	1107-1110 bp	1.0	78.3
trnC-GCA-petN_p3	GACATAGCCAAGCGGTAAGG	AGTCCACTCCTTCCCCATAC	55.0	55.0	58.4	58.1	1144-1147 bp	1.0	77.8
trnC-GCA-petN_p4	GACATAGCCAAGCGGTAAGG	GTCCACTCCTTCCCCATACT	55.0	55.0	58.4	58.1	1143-1146 bp	1.0	77.8
trnC-GCA-petN_p5	GACATAGCCAAGCGGTAAGG	AGCCCAAGCGAGACTTACTA	55.0	50.0	58.4	58.2	1085-1088 bp	1.0	77.7