Hotspot

ndhF

SSC · 114975-117206 · 2232 bp

Back to Combretum

Pi 0.0197

MarkerSeek score 54.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 44
pi: 0.0197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGGACGAAACAGGAACAAGA	AGATAAGAGCCGCGGAATTG	50.0	50.0	57.8	58.1	2724 bp	0.5	57.0
ndhF_p2	GGGACGAAACAGGAACAAGA	GATAAGAGCCGCGGAATTGA	50.0	50.0	57.8	58.1	2723 bp	0.5	56.9
ndhF_p3	ACGAAACAGGAACAAGAGGG	AGATAAGAGCCGCGGAATTG	50.0	50.0	57.7	58.1	2721 bp	0.5	56.9
ndhF_p4	GGAACAAGAGGGATTCACCG	AGATAAGAGCCGCGGAATTG	55.0	50.0	58.3	58.1	2713 bp	0.5	56.9
ndhF_p5	ACGAAACAGGAACAAGAGGG	GATAAGAGCCGCGGAATTGA	50.0	50.0	57.7	58.1	2720 bp	0.5	56.9