Hotspot

rpl32-JXY33_pgt031

SSC · 118020-118422 · 403 bp

Back to Combretum

Pi 0.0578
MarkerSeek score 80.20
Primer available yes
Label rpl32-JXY33_pgt031

Feature Metadata

label_name
rpl32-JXY33_pgt031
feature_type
igs
region
SSC
length_bp
403
variable_sites
23
pi
0.0578
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-JXY33_pgt031_p1 GGCGTTGAAAGCTTTTTCGT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 526-770 bp 1.0 82.1
rpl32-JXY33_pgt031_p2 GGCGTTGAAAGCTTTTTCGT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 527-771 bp 1.0 82.1
rpl32-JXY33_pgt031_p3 GGCGTTGAAAGCTTTTTCGT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 536-780 bp 1.0 81.1
rpl32-JXY33_pgt031_p4 GGCGTTGAAAGCTTTTTCGT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.2 57.8 535-779 bp 1.0 80.5
rpl32-JXY33_pgt031_p5 GGCGTTGAAAGCTTTTTCGT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.2 57.8 534-778 bp 1.0 80.5