Hotspot

rpl20-rps12

LSC · 72827-73600 · 774 bp

Back to Combretum

Pi 0.0275

MarkerSeek score 75.40

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 21
pi: 0.0275
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	GTAAGTCTTGAACGAGCCCC	CGCCGAGGAACATGTACTAG	55.0	55.0	58.3	57.9	896-945 bp	1.0	79.4
rpl20-rps12_p2	TTTGTTCTACGTCTCCGAGC	CGCCGAGGAACATGTACTAG	50.0	55.0	57.7	57.9	848-897 bp	1.0	79.0
rpl20-rps12_p3	TGTTCTACGTCTCCGAGCTA	CGCCGAGGAACATGTACTAG	50.0	55.0	57.6	57.9	846-895 bp	1.0	78.7
rpl20-rps12_p4	TTGTTCTACGTCTCCGAGCT	CGCCGAGGAACATGTACTAG	50.0	55.0	58.5	57.9	847-896 bp	1.0	78.0
rpl20-rps12_p5	CCCGAAAGCTTGATGCAAAT	CGCCGAGGAACATGTACTAG	45.0	55.0	57.3	57.9	877-926 bp	1.0	76.4