Hotspot

accD-psaI

LSC · 62168-62800 · 633 bp

Back to Combretum

Pi 0.0202

MarkerSeek score 73.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 633
variable_sites: 12
pi: 0.0202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCCAATCGTACCACGTAATCC	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	806-848 bp	1.0	73.1
accD-psaI_p2	CCAATCGTACCACGTAATCCT	CCATTGCAATTGCCGGAAAT	47.6	45.0	57.6	58.0	805-847 bp	1.0	73.0
accD-psaI_p3	TCCAATCGTACCACGTAATCC	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.6	57.9	812-854 bp	1.0	72.4
accD-psaI_p4	CCAATCGTACCACGTAATCCT	AAGAAGCCATTGCAATTGCC	47.6	45.0	57.6	57.9	811-853 bp	1.0	72.4
accD-psaI_p5	AAGGGTGTTCTGAGTGAGTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	56.3	58.0	781-823 bp	1.0	71.2