Hotspot

JXY33_pgt011-psbD

LSC · 34366-35806 · 1441 bp

Back to Combretum

Pi 0.0491

MarkerSeek score 71.90

Primer available yes

Label JXY33_pgt011-psbD

Feature Metadata

label_name: JXY33_pgt011-psbD
feature_type: igs
region: LSC
length_bp: 1441
variable_sites: 56
pi: 0.0491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 71.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
JXY33_pgt011-psbD_p1	ACTGGGAAAGGGGATCAAGA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1763-1986 bp	1.0	86.3
JXY33_pgt011-psbD_p2	ACTGGGAAAGGGGATCAAGA	AAAGGTTGTACCCGTGAACC	50.0	50.0	57.9	58.0	1844-2067 bp	1.0	86.0
JXY33_pgt011-psbD_p3	ACTGGGAAAGGGGATCAAGA	CGGAATGTATTTGCGCCATC	50.0	50.0	57.9	58.0	2380-2603 bp	1.0	85.4
JXY33_pgt011-psbD_p4	TGATTCAGCTGTTCCGGAAG	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	2134 bp	0.5	59.2
JXY33_pgt011-psbD_p5	GCCCTTTTAACTCAGCGGTA	GAAACGGTCCCTACGTAACC	50.0	55.0	57.9	58.0	1596 bp	0.5	59.1