Hotspot

petN-psbM

LSC · 30797-31759 · 963 bp

Back to Combretum

Pi 0.0299

MarkerSeek score 73.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 963
variable_sites: 27
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTCT	45.0	42.9	58.0	57.0	1104-1118 bp	1.0	70.1
petN-psbM_p2	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.8	57.0	1110-1124 bp	1.0	68.7
petN-psbM_p3	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTCTTCATTCT	50.0	42.9	57.6	57.0	1050-1064 bp	1.0	67.5
petN-psbM_p4	TCTTGCTTGGGCTGCTTTAA	TGCTACTGCACTCTTCATTC	45.0	45.0	58.0	55.5	1104-1118 bp	1.0	65.8
petN-psbM_p5	AGTAAGTCTTGCTTGGGCTG	TGCTACTGCACTCTTCATTC	50.0	45.0	57.8	55.5	1110-1124 bp	1.0	64.3