Hotspot

rps16-JXY33_pgt003

LSC · 6363-7641 · 1279 bp

Back to Combretum

Pi 0.234

MarkerSeek score 77.70

Primer available yes

Label rps16-JXY33_pgt003

Feature Metadata

label_name: rps16-JXY33_pgt003
feature_type: igs
region: LSC
length_bp: 1279
variable_sites: 139
pi: 0.234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-JXY33_pgt003_p1	CCACAGCTGATTATGTCCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.7	58.1	706-1373 bp	1.0	88.9
rps16-JXY33_pgt003_p2	ATTATGTCCGTCAAGTCGCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	697-1364 bp	1.0	87.8
rps16-JXY33_pgt003_p3	GCGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.5	58.1	678-1345 bp	1.0	87.2
rps16-JXY33_pgt003_p4	TCCACAGCTGATTATGTCCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.4	58.1	707-1374 bp	1.0	86.6
rps16-JXY33_pgt003_p5	CCACAGCTGATTATGTCCGT	GGGTTTTGGTCCCGGTATTC	50.0	55.0	57.7	58.5	727-1394 bp	1.0	85.2