Hotspot

trnH-psbA

LSC · 77-677 · 601 bp

Back to Combretum

Pi 0.0656

MarkerSeek score 63.30

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 601
variable_sites: 17
pi: 0.0656
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.43
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CTGCACGTGAACTAGGTGTT	TCAGTGCTATGCATGGTTCC	50.0	50.0	58.1	58.0	1103 bp	0.5	58.6
trnH-psbA_p2	CTGCACGTGAACTAGGTGTT	CAGTGCTATGCATGGTTCCT	50.0	50.0	58.1	58.0	1102 bp	0.5	58.5
trnH-psbA_p3	CTGCACGTGAACTAGGTGTT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.1	57.9	717 bp	0.5	58.4
trnH-psbA_p4	CTGCACGTGAACTAGGTGTT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	58.1	57.9	720 bp	0.5	58.3
trnH-psbA_p5	CTGCACGTGAACTAGGTGTT	CGGATGGTATGCCTCTAGGA	50.0	55.0	58.1	58.1	1229 bp	0.5	58.3