Hotspot

petB

LSC · 73224-74574 · 1351 bp

Back to Comastoma

Pi 0.00148

MarkerSeek score 59.60

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1351
variable_sites: 2
pi: 0.00148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGAGTTCGACCGTGGAATTT	TCCCCTGAGTGACAAATGGA	50.0	50.0	58.2	58.3	1464 bp	1.0	76.5
petB_p2	GGAGTTCGACCGTGGAATTT	TTCCCCTGAGTGACAAATGG	50.0	50.0	58.2	57.4	1465 bp	1.0	73.9
petB_p3	GGAGTTCGACCGTGGAATTT	AGTACTTCCCCTGAGTGACA	50.0	50.0	58.2	57.3	1470 bp	1.0	73.0
petB_p4	TTGTTTTGGGAGTTCGACCG	TCCCCTGAGTGACAAATGGA	50.0	50.0	58.7	58.3	1472 bp	1.0	72.4
petB_p5	GGAGTTCGACCGTGGAATTT	CCCCTGAGTGACAAATGGAT	50.0	50.0	58.2	57.2	1463 bp	1.0	72.0