Hotspot

atpI

LSC · 13284-14024 · 741 bp

Back to Comastoma

Pi 0.00135

MarkerSeek score 59.50

Primer available yes

Label atpI

Feature Metadata

label_name: atpI
feature_type: gene
region: LSC
length_bp: 741
variable_sites: 1
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI_p1	TTGTCGTGAACCAAACGTCT	AGGCAAAGAGATGGTTGAATCA	45.0	40.9	58.0	58.0	843 bp	1.0	63.7
atpI_p2	GTCGTGAACCAAACGTCTCT	AGGCAAAGAGATGGTTGAATCA	50.0	40.9	58.2	58.0	841 bp	1.0	62.6
atpI_p3	TTGTCGTGAACCAAACGTCT	AGTAGGCAAAGAGATGGTTGA	45.0	42.9	58.0	56.6	846 bp	1.0	61.1
atpI_p4	TTGTCGTGAACCAAACGTCT	TCAAGTAGGCAAAGAGATGGT	45.0	42.9	58.0	56.6	849 bp	1.0	61.1
atpI_p5	TGTCGTGAACCAAACGTCTC	AGGCAAAGAGATGGTTGAATCA	50.0	40.9	58.4	58.0	842 bp	1.0	60.3