Hotspot

trnL-CAA-ycf15

IRa · 141694-142099 · 406 bp

Back to Comastoma

Pi 0.00985

MarkerSeek score 60.40

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 406
variable_sites: 4
pi: 0.00985
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	AACAGCGAACAACCCATTCA	50.0	45.0	58.0	58.3	541 bp	1.0	78.0
trnL-CAA-ycf15_p2	AAATGGTAGACACGCGAGAC	TGAACAGCGAACAACCCATT	50.0	45.0	58.0	58.3	543 bp	1.0	78.0
trnL-CAA-ycf15_p3	AAATGGTAGACACGCGAGAC	TTGAACAGCGAACAACCCAT	50.0	45.0	58.0	58.3	544 bp	1.0	78.0
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	AACAGCGAACAACCCATTCA	50.0	45.0	58.0	58.3	543 bp	1.0	78.0
trnL-CAA-ycf15_p5	TGAAATGGTAGACACGCGAG	TGAACAGCGAACAACCCATT	50.0	45.0	58.0	58.3	545 bp	1.0	78.0