Hotspot

ycf15-trnL-CAA

IRb · 91369-91774 · 406 bp

Back to Comastoma

Pi 0.00985
MarkerSeek score 60.40
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
406
variable_sites
4
pi
0.00985
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 AACAGCGAACAACCCATTCA AAATGGTAGACACGCGAGAC 45.0 50.0 58.3 58.0 541 bp 1.0 78.0
ycf15-trnL-CAA_p2 TGAACAGCGAACAACCCATT AAATGGTAGACACGCGAGAC 45.0 50.0 58.3 58.0 543 bp 1.0 78.0
ycf15-trnL-CAA_p3 TTGAACAGCGAACAACCCAT AAATGGTAGACACGCGAGAC 45.0 50.0 58.3 58.0 544 bp 1.0 78.0
ycf15-trnL-CAA_p4 AACAGCGAACAACCCATTCA TGAAATGGTAGACACGCGAG 45.0 50.0 58.3 58.0 543 bp 1.0 78.0
ycf15-trnL-CAA_p5 TGAACAGCGAACAACCCATT TGAAATGGTAGACACGCGAG 45.0 50.0 58.3 58.0 545 bp 1.0 78.0