Hotspot

ndhC-trnV-UAC

LSC · 49238-49884 · 647 bp

Back to Comastoma

Pi 0.00155
MarkerSeek score 59.60
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
647
variable_sites
1
pi
0.00155
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CGGGTCCTTTCCTAATTGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 57.9 57.8 795-847 bp 1.0 78.1
ndhC-trnV-UAC_p2 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 778-830 bp 1.0 77.4
ndhC-trnV-UAC_p3 AATTGGGGCTAAAACTCCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.2 57.8 782-834 bp 1.0 77.3
ndhC-trnV-UAC_p4 GGTCCTTTCCTAATTGGGGC AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.2 57.8 793-845 bp 1.0 76.7
ndhC-trnV-UAC_p5 CGGGTCCTTTCCTAATTGGG GAAGGTCTACGGTTCGAGTC 55.0 55.0 57.9 57.5 794-846 bp 1.0 75.3