Hotspot

trnT-UGU-trnL-UAA

LSC · 45178-46032 · 855 bp

Back to Comastoma

Pi 0.00585

MarkerSeek score 54.40

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 5
pi: 0.00585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	55.0	50.0	57.9	58.1	913-920 bp	1.0	78.8
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	50.0	50.0	57.8	58.1	945-952 bp	1.0	77.9
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	919-926 bp	1.0	77.5
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	951-958 bp	1.0	76.6
trnT-UGU-trnL-UAA_p5	GCGATGCTCTAACCTCTGAG	TACCAATTTCGCCATACCCC	55.0	50.0	57.9	57.6	906-913 bp	1.0	76.6