Hotspot

trnR-UCU-atpA

LSC · 8738-8893 · 156 bp

Back to Comastoma

Pi 0.0192

MarkerSeek score 63.60

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 156
variable_sites: 3
pi: 0.0192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AGGGATAAAAGGCGTCCATT	GGCGCAAGTCCTTTTGAAAG	45.0	50.0	56.9	58.2	309 bp	1.0	70.0
trnR-UCU-atpA_p2	GGGATAAAAGGCGTCCATTG	GGCGCAAGTCCTTTTGAAAG	50.0	50.0	56.8	58.2	308 bp	1.0	69.7
trnR-UCU-atpA_p3	AGGGATAAAAGGCGTCCATT	AAACATTTACCGAGGAGGCG	45.0	50.0	56.9	58.3	325 bp	1.0	69.6
trnR-UCU-atpA_p4	GGGATAAAAGGCGTCCATTG	AAACATTTACCGAGGAGGCG	50.0	50.0	56.8	58.3	324 bp	1.0	69.4
trnR-UCU-atpA_p5	AGGGATAAAAGGCGTCCATTG	GGCGCAAGTCCTTTTGAAAG	47.6	50.0	58.3	58.2	309 bp	1.0	68.2