Hotspot

trnK-UUU-trnQ-UUG

LSC · 4285-5830 · 1546 bp

Back to Comastoma

Pi 0.00182

MarkerSeek score 48.90

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1546
variable_sites: 2
pi: 0.00182
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AGTCGTGGTCTTGCAAACTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.0	58.1	1243-1687 bp	1.0	83.2
trnK-UUU-trnQ-UUG_p2	GGACGAATCCCTTGCTTCAT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1211-1655 bp	1.0	83.1
trnK-UUU-trnQ-UUG_p3	AGTCGTGGTCTTGCAAACTT	GCTACTCGGAGGTTCGAATC	45.0	55.0	58.0	57.9	1251-1695 bp	1.0	82.8
trnK-UUU-trnQ-UUG_p4	GGACGAATCCCTTGCTTCAT	GCTACTCGGAGGTTCGAATC	50.0	55.0	58.0	57.9	1219-1663 bp	1.0	82.8
trnK-UUU-trnQ-UUG_p5	AAACTTTACCGACGGTCTGG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	1229-1673 bp	1.0	82.2