Hotspot

ycf1

SSC · 125309-130936 · 5628 bp

Back to Coluria

Pi 0.0215

MarkerSeek score 50.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5628
variable_sites: 121
pi: 0.0215
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTAATAAAAGGGGCCGCTCC	TCGATTAATCCGCAAATCCCA	50.0	42.9	57.9	57.8	387 bp	1.0	73.2
ycf1_p2	GGCCGCTCCGTTATATTGAA	TCGATTAATCCGCAAATCCCA	50.0	42.9	58.1	57.8	376 bp	1.0	72.8
ycf1_p3	TTAATAAAAGGGGCCGCTCC	ACACAACCAGCTCTTGAATCA	50.0	42.9	57.9	57.8	290 bp	0.5	49.8
ycf1_p4	TTAATAAAAGGGGCCGCTCC	GGGATTTCTAAGTCGAGAGCG	50.0	52.4	57.9	58.3	495 bp	0.5	49.4
ycf1_p5	GGCCGCTCCGTTATATTGAA	ACACAACCAGCTCTTGAATCA	50.0	42.9	58.1	57.8	279 bp	0.5	49.3